Metabarcoding results from the 16S marker (English, ZIP)

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2026-04-08

The Eastern Shore Islands Area of Interest (AOI) is a large coastal AOI in the Maritimes Region spanning 2089 km2 and extending along 100 km of coastline. While much of the data that led to the area's designation as an AOI was collected in the nearshore coastal areas (i.e., seagrass and kelp distributions, coastal bird nesting sites, herring spawning areas), there is less information available on the fish and invertebrate community structure in offshore areas of the AOI. Current offshore sampling effort is primarily focused in the Summer multi-species RV survey which covers the Scotian Shelf but does not sample within the depth range covered by the AOI (~ <100m depth). Environmental DNA (eDNA) offers a promising approach for biodiversity monitoring that has been gaining increased attention in the marine realm. Through sampling relatively small volumes of water (~1-5 L), eDNA can capture of millions of fragments of eDNA on filters that can then be sequenced to identify the species composition of an area. eDNA metabarcoding is based on the foundations of DNA barcoding, where species are identified solely by their unique DNA sequences. eDNA metabarcoding relies on a comprehensive…

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