View ECCC Data Mart (English) (English, HTML)

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Publisher-reported update

2023-12-17

This study aimed to address the toxicological effects the type-2 diabetes drug, metformin has on wild fathead minnows (Pimephales promelas). Fathead minnow embryos and adults were exposed to metformin via in-lake mesocosm and their survival, development and growth was assessed. Exposures were conducted at Lake 114 located at the International Institute for Sustainable Development-Experimental Lakes Area (IILSD-ELA) in northern Ontario, Canada. Adults were exposed for 56 days to three concentrations of metformin (0, 4, and 40 micrograms per liter (ug/L)) each with four replicates. Fish behavior and mortality was observed daily during the exposure period. After 56 days, fish were captured, dissected and assessed for various health endpoints. Additionally, blood, brain and tissue samples were collected for metabolic endpoints. Additionally, wild fathead minnow eggs spawned in the lake were collected in glass mason jars and transported back to the on site laboratory. One fertilized egg was placed in each well for a total of 20 eggs per plate (thus 20 eggs per replicate for the 3 replicates for a total of 60 eggs per mesocosm and a total of 240 eggs per treatment). Hatching time,…

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