Scientific Publication - Effects of multi-well plate incubation on embryo-larval development in the fathead minnow (Pimephales promelas) (English, HTML)

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2018-09-12

Fathead minnow (Pimephales promelas) embryos and larvae are frequently used in toxicology, including short-term embryo-only tests which often use small volumes of test solution. The effect that such conditions may have on Fathead minnow development has yet to be explicitly described. Here we compared rates of embryonic development in Fathead minnow embryos reared under standard light and temperature conditions with a range of possible methods. All methods yielded excellent control survival. We demonstrated that Fathead minnow embryos incubated in a range of small volumes in multi-well plates [500 microlitres (μL) to 2 millilitres (mL) per embryo] did not substantially vary in developmental rate, but flexed less frequently as embryos, hatched smaller, later and with larger yolk-sacs, and initiated feeding later than embryos reared in an excess of solution (20 mL per embryo) with or without supplemental aeration. Faster hatch and growth were promoted with an orbital shaker, but growth benefits were not sustained into the larval stage. Developmental differences persisted in larvae reared to 20 days post-fertilization when monitoring ceased, but growth differences did not magnify and…

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